Sanger Sequencing

Sanger sequencing (also known as dideoxy or capillary electrophoresis sequencing) is an excellent choice when a small region of DNA to be analyzed and looking for a fast and cost-effective sequencing.
Our lab is fully equipped with expert staff to handle DNA sequencing projects of various complexities including PCR product sequencing, plasmid DNA sequencing, Sequencing of other DNA constructs, direct bacterial colony sequencing, Sequencing of glycerol stock, GLP DNA sequencing.
Our specialty is to provide cutting-edge MDx grade Sanger sequencing and data analysis services with high quality and accuracy to Molecular Diagnostic labs (MDx labs).
We offer DNA sequencing read lengths of up to ~900-1100 bases (Phred20 score).
Our Comprehensive Sanger sequencing Portfolio
  • Routine single or bidirectional sequencing on PCR products and plasmid DNA of single samples or plates.
  • Primer walking services with a guaranteed final data accuracy of > 99.90%.
  • Molecular identification services of bacteria (16SrRNA gene) and fungi (18S/ITS/26S rRNA).
  • DNA Barcoding services for Insect, Animal and plants (COI/COII/matK etc).
  • Single nucleotide polymorphism (SNP) Genotyping services through PCR and Sanger sequencing.
  • Targeted re-sequencing of defined genomic regions after Next Generation Sequencing(NGS).
  • Cloning of PCR products and subsequent sequencing.
  • Primer design, synthesis and storage.

Primer Walking Services

Primer Walking is used to fill in the gaps and give a full sequence or additional coverage as needed.
Siriprogen’s Primer walking services offers an option to choose single-stranded (SS) and double-stranded (DS) sequencing for DNA templates that are longer than 1400 bases. Our Primer walking services offers to discover unknown regions.

High Quality

A guaranteed final data accuracy of ≥ 99.90%.

Fast

Up to 1600 base pairs per day (even faster when a reference sequence is available).

Deliverables

Delivery of electronic files such as project data sheet including sequencing strategy, text files and chromatograms (.ab1 and .pdf format) for all the reactions. Consensus sequence as .FASTA file.

Deliverables: Delivery of electronic files such as project data sheet including sequencing strategy, text files and chromatograms (.ab1 and .pdf format) for all the reactions. Consensus sequence as .FASTA file.

Primer Walking Process

1.
Submit Samples
2.
Primer Design
3.
Sanger Sequencing
4.
Contig Generation
5.
Report
1.
Submit Samples
2.
Primer Design
3.
Sanger Sequencing
4.
Contig Generation
5.
Report

Molecular Identification Services

Molecular identification service is used to identify various isolates of microbes (Bacteria and Fungi). It is not only an alternative to traditional phenotypic detection methods whilst it offers highly sensitive, specific and fast in detecting slow growing and non-culturable organisms.
Siriprogen, provides fast and accurate identification of bacteria (16S rRNA gene) and fungi (18S/ITS/26S rRNA) by DNA sanger sequencing method.
We provide complete service which includes Nucleic acid isolation, PCR Amplification, Sanger sequencing, Contig generation and Report.
BLAST analysis of contig sequence was performed with the database of NCBI Genbank. Based on maximum identity score, top ten sequences were selected and aligned using multiple sequence alignment software (MAS), such as “CLUSTALW.” Distance matrix was generated and the Phylogenetic tree was constructed using bioinformatics tools.
Deliverables 
This service includes sequencing of ribosomal genes and/or other conserved region and its comprehensive report which includes genus and species level identification (if possible) along with phylogenetic tree.
We accept the below mentioned starting materials for this
  • Bacterial and fungal colonies.
  • Extracted gDNA.
  • Glycerol stocks.
  • Cell pellets.

Barcoding Services

DNA Barcoding is a method for species identification that uses a short DNA sequence in a specific gene or genes of an organism.

Bacteria, Actinomycetes, Fungi and Algae

Plant Barcoding

Land vertebrates, Fishes and Seafood

Insect Barcoding (termites, spiders etc.)

Barcoding Process

1.
Unknown Species
2.
DNA Extraction
3.
DNA Amplifiation
4.
Sanger Sequencing
5.
Matching Sequence
6.
Report
1.
Unknown Species
2.
DNA Extraction
3.
DNA Amplification
4.
Sanger Sequencing
5.
Matching Sequence
6.
Report

SNP Genotyping Services

Genotyping is a method of determining differences in the genetic make-up (genotype) of an organism. This method does this by comparing the individual’s DNA sequence against a reference sequence.
SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic aberrations among all the other types of aberrations.
Siriprogen Life Sciences, SNP Genotyping service is a PCR and Sanger sequencing-based solution that is used to SNP screening assays and validate SNPs of interest with speed and accuracy.
Deliverables  SNP Genotyping projects will receive a chromatogram file (.ab1 and .pdf) along with a final report identifying SNPs compared to the provided reference sequence. Custom reports are also available upon request.